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goat anti 5 lipoxygenase  (Novus Biologicals)


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    Structured Review

    Novus Biologicals goat anti 5 lipoxygenase
    Goat Anti 5 Lipoxygenase, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+5+lipoxygenase/5-Lipoxygenase+Antibody/pmc11310472-296-12-19
    Average 92 stars, based on 3 article reviews
    goat anti 5 lipoxygenase - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h.

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors.
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h. Organ bath force recording Whole gallbladders were harvested and placed in minimum essential medium (MEM; Invitrogen Gibco, Oslo, Norway).

    Immunofluorescence:

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h.

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors.
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h. Organ bath force recording Whole gallbladders were harvested and placed in minimum essential medium (MEM; Invitrogen Gibco, Oslo, Norway).

    Incubation:

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h.

    Article Title: Bitter tastants relax the mouse gallbladder smooth muscle independent of signaling through tuft cells and bitter taste receptors.
    Article Snippet: Native fluorescence was documented with a Zeiss Imager M2 microscope (Zeiss, Oberkochem, Germany), equipped with a Zeiss AxioCam HRc camera, and Zeiss ZEN 2011 software. .. To combine the visualization of native mCherry fluorescence with single immunofluorescence, a goat anti-5 lipoxygenase-activating protein (FLAP, NB300-891, 1:50; Novus Biol., Centennial, CO, USA) antiserum was applied in PBS/1% BSA and incubated overnight at 16 °C, followed by 2 h at 37 °C. .. After extensive washing in distilled water followed by PBS, immunoreactions were visualized by a two-step procedure using a species-specific biotinylated secondary antibody (703-065-155, 1:200, Jackson ImmunoResearch), applied for 45 min, followed by streptavidin conjugated with Alexa Fluor 488 (016-160-084, 1:200, Jackson ImmunoResearch) for 2 h. Organ bath force recording Whole gallbladders were harvested and placed in minimum essential medium (MEM; Invitrogen Gibco, Oslo, Norway).



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